In this study, we investigated the relationship of chemical stressors, that are a arousal and a depressant on the nervous im pulsate counts of a Lumbriculus variegates worm, which was used during the lab. The worms were exposed to chemical stressors as mentioned before, which were a stimulative and a depressant, to determine the effect on its pulse rate. The results that were obtained in the lab showed that caffeine, which was a stimulant, induced an increase in the pulse rate of the Lumbriculus variegates worm, scarce ethanol didnt induce an increase in the pulse rate of the Lumbriculus variegates worm. The worm was placed on a slide under the microscope, for it to be observed at 4x objective.
INTRODUCTION
Lumbriculus variegatus is a freshwater oligochaete worm that tolerate be found in North America in shoal habitats at the edge of ponds, lakes, and marshes. Not only is it found there, but also in the sediments as well. The worm has a dilute outer body wall and is able to breathe by diffusion of gases across the body wall near the tail. It can produce asexually, or sexually. It has a closed circulatory system, which contains brawny blood vessels pumping fluid through the system.
In this study, the main subject that it is going to be determined is whether a stimulant (caffeine) or a depressant (ethanol) will affect the pulse rate of the worm.
The stimulant would have more effect on the pulse rate of the worm, because the effect would occur faster than the depressant.
MATERIALS AND METHODS
1) Organism
Lumbriculus variegates was a worm that was used in this experiment, as it was observed under a microscope using the 4x objective.
2) Chemical
Ethanol was used in this experiment as a solution that was added to the worm so that the effect on its pulse rate would be established. The chemicals were prepared that stimulates either harmonic or parasympathetic nervous systems.
3) Test set-up
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